Laboratory of Soil Science and Plant Nutrition

Mie University                                Last update 06/Oct/2015

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Welcome to our homepage. Dr. Mizuno ‘s labo. Standing on the metal analysis, we are studying about these theme.

”survival strategy of plans on metal-rich conditions and biological diversity”

“greening of serpentine and limestone quarry areas with wild thyme”

From 2014, our laboratory has not performed gene modifining or any molecular analyses, and mainly investigated metal concentration in plant tissues, and research on plant diversity on the serpentine soil.

PI: Associate Prof. Takafumi Mizuno
born at 16/Aug/1969
Dr. of Agriculture (Hokkaido Univ.)

Papers (I can send you some papers in PDF files. Please do not hesitate for request)

Nishida S, Kato A, Tsuzuki C, Yoshida J, Mizuno T.  Induction of nickel accumulation in response to zinc deficiency in Arabidopsis thaliana. International Journal of Molecular Sciences, 16, 9420-9430 (2015)  doi:10.3390/ijms16059420 

Takafumi Mizuno and Yoko Kirihata, Elemental composition of plants from the serpentine soil of Sugashima Island, Japan. Australian Journal of Botany, Accepted (Dec.3)

Mizuno T, Emori K and Ito S.  Manganese hyperaccumulation from no-contaminated soil in Chengiopanax sciadophylloides and its correlation with calcium accumulation. Soil Science and Plant Nutrition. 59, 591-602


Nishida S,  Aisu A,,and Mizuno T, Induction of IRT1 by the nickel-induced iron-deficient response in Arabidopsis.

Plant Signaling and Behavior . 7, 1-3 (2012)

Harada E, Hokura A, Nakai I, Terada Y, Baba K, Yazaki K, Shiono M, Mizuno N and Mizuno T, Assessment of willow (Salix sp.) as a woody heavy metal accumulator: field survey and in vivo X-ray analyses. Metallomics, 3,1340-1346 (2011)

Nishida S, Tsuzuki C, Kato A, Aisu A, Yoshida J and Mizuno T. AtIRT1, the Primary Iron Uptake Transporter in the Root,

Mediates Excess Nickel Accumulation in Arabidopsis thaliana. Plant Cell Physiol. 52(8): 1433-1442, 2011

Fernando DR,  Mizuno T, Woodrow IE, Baker AJM and  Collins RN. Characterisation of foliar Mn in Mn-(hyper)accumulators using X-ray absorption spectroscopy. New Phytologist, 188: 1014–1027 (2010)

Nishida S, Morinaga Y, Obata H and Mizuno T. 2011. Identification of the N-terminal Region of TjZNT2, a Zrt/Irt-like Protein Family Metal Transporter, as a Novel Functional Region Involved in Metal Ion Selectivity. FEBS Journal, 278, 851-858,

Takafumi Mizuno,, Masahiro Nakagawa, Hiroshi Ono, Daisuke Sugiura, Hideo Tamura, Hitoshi Obata. Isolation of multidrug resistance associated protein like gene from lead hyperaccumulator common buckwheat and its lead detoxification ability. Plant Biotechnology, 27,39-46 (2010)

Mizuno T, Horie K, Nosaka S, Obata H,and  Mizuno N. Serpentine plants in Hokkaido and their chemical characteristics. Northeastern Naturalist. 16, 65-80 (2009)

Mizuno T, Asahina R, Hosono A, Tanaka A, Senoo K and Obata H. Age-dependent manganese hyperaccumulation in Chengiopanax sciadophylloides (Araliaceae). Journal of Plant Nutrition. 31, 1811–1819 (2008)

Sutigoolabud P, Mizuno T, Ongprasert S, Karita S, Takahashi T, Obata H and Senoo K. Effect of chlorate on nitrification  in longan plantation soil. Soil Sci. Plant Nutr. 54, 387–392 (2008)

Nishida S, Mizuno T, and Obata H. Involvement of histidine-rich domain of ZIP family transporter TjZNT1 in metal ion specificity. Plant Physiol. Biochem. 46 ,601-606 (2008).

Mizuno N, Amano Y, Mizuno T,  and Nanzyo M. Changes in the heavy minerals content and the element concentration of Tarumae-a tephra with distance from the source volcano.  Soil Sci. Plant Nutr. 54, 839–845(2008).

Takahashi T, Furuichi Y, Mizuno T, Kato M, Tabara A, Kawada Y, Hirano Y, Kubo K, Onozuka M and Kurita O. Water-holding capacity of insoluble fibre decreases the free water and elevates digesta viscosity in the rat.J. Sci .Food.Agric. 89: 245 – 250 (2009)

Obata H., Asahina R, and Mizuno T. Heavy metal concentration of Plant leaves at three sites in Mie Prefecture. Bull. Fac. Bioresources Mie Univ. 35,55-63

Mizuno T, Usui K, Nishida S, Unno T, and Obata H. Investigation of the basis for Ni tolerance conferred by the expression of TjZnt1 and TjZnt2 in yeast strains. Plant Physiol. Biochem. 45(6-7), 371-378(2007).

Mizuno T, Hirano K, Kato S and Obata H. Cloning of ZIP family metal transporter genes from a Mn hyperaccumulator plant Chengiopanax sciadophylloides, and its metal transport and resistance abilities in yeast.  Soil Sci. Plant Nutr. 54(1), 86-94 (2008)

Mizuno, T., Sonoda,T., Sugiura, D. and Obata , H.Expression of Thlaspi caerulescens phytochelatin synthase gene and its role in Cd tolerance in yeast. Bull. Fac. Bioresources Mie Univ. 34,27-36 (2007).

Mizuno T, Hirano K, Hosono A, Kato S, and Obata H. Continual pH lowering and manganese dioxide solubilization

in the rhizosphere of Mn-hyperaccumulator plant Chengiopanax sciadophylloides. Soil Sci. Plant Nutr.52(6), 726-733(2006)

Sutigoolabud, P., Senoo, K., Ongprasert, S., Mizuno, T., Mishima, T., Hisamatsu, M. and Obata, H. Decontamination of Chlorate in Longan Plantation Soils by Bio-Stimulation with Molasses Amendment. Soil Sci. Plant Nutr. 51(4) 583-588(2005)

Mizuno, T., Obata, H., Horie, K., Nosaka, S., and Mizuno, N Comparison of Ni/Zn metal accumulation ability of Thlaspi japonicum from three different areas in Hokkaido. Soil Sci. Plant Nutr. 51(4) 589-594(2005)

Mizuno T, Usui K, Horie K, Nosaka S, Mizuno N, and Obata H. Cloning of three ZIP/Nramp transporter genes from a Ni hyperaccumulator plant Thlaspi japonicum and their Ni2+-transport abilities. Plant Physiol. Biochem. 43(8), 793-801 (2005)

Mizuno, T., and Obata, H., Ni transporting function of ZIP/Nramp transporters in Ni hyperaccumulator plant Thlaspi japonicum. C. J. Li et al. (Eds), Plant Nutrition for food security, human health and environmental protection. 672-673, Tsinghua University Press(2005)

Sutigoolabud, P., Senoo, K, Ongprasert, S, Mizuno, T, Tanaka, A, Obata, H and Hisamatsu, M. Decontamination of Chlorate in Longan Plantation Soils by Bio-stimulation with Sugar Amendment. Soil Sci. Plant Nutr. 50(2) 249-256(2004)

Tanaka, A., Sugimoto, H., Muta, Y., Mizuno, T., Senoo, K., Obata., H and Inoue, K. DSC Studies on the Effects of Ca2+ on the Thermal Unfolding Pseudomonas cepacia Lipase. Bioscience, Biotechnology and Biochemistry 67 (1), 207-210 (2003)

Mizuno, N.Nosaka, S., Mizuno, T., Horie, K., and Obata, H. Distribution of Ni and Zn in the leaves of Thlaspi japonicum growing on ultramafic soil. Soil Sci. Plant Nutr. 49(1), 93-97(2003)

Wang, Z., Kontani, Y., Sato Y., Mizuno, T., Mori, N. and Yamashita, H. Muscle type difference in the regulation of UCP3 under cold conditions. Biochemical and Biophysical Research Communications, 305(2), 244-249 (2003)

Mizuno, T.,Sonoda, T., Horie, K.,  Senoo, K., Tanaka, A., Mizuno, N., and Obata, H. Cloning and characterization of phytochelatin synthase from a nickel hyperaccumulator Thlaspi japonicum and its expression in yeast. Soil Sci. Plant Nutr. 49(2), 285-290(2003)