Single conidium isolation:  Using a flame-sterilized micro-spatula, conidia are collected from caespituli or from affected spots of the leaves and suspended in sterilized water on a microscope slide. The conidial suspension is further diluted in sterilized water and pipet onto a 2% aqueous agar in a Petri dish (9 cm in diam.), and spread over the surface using a flame-sterilized micro-spatula. After incubation at 20 ºC in the dark for 24 h, the germinating conidia will be individually transferred onto MEA using a flame-sterilized micro-tube under a light microscope.

Nakashima et al. 2011. Addition and reexamination of Japanese species belonging to the genus Cercospora and allied genera. X. Newly recorded species from Japan (5). Mycoscience 52:253-259.

doi:10.1007/s10267-010-0094-z