研究テーマ概要
糸状菌(カビ)のセルロース系バイオマス分解酵素遺伝子の発現制御機構の解明とその応用をテーマに研究しています。糸状菌は多種類のセルラーゼやヘミセルラーゼを作るため、バイオマスの効率的な分解に有用ですが、産業利用するには十分ではありません。遺伝子発現制御機構を解明して、転写活性化因子の改良やカーボンカタボライト抑制機構を抑えることができれば、セルラーゼやヘミセルラーゼ遺伝子の転写を包括的に増大させることができ、複数の酵素の同時大量生産につながります。

研究報文

Tani, S., Yuki, S., Kunitake, E., Sumitani, J. and Kawaguchi, T, Dipeptidyl peptidase IV is involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes in Aspergillus aculeatus Biosci Biotechnol Biochem 81(6) 1227-1234 (2017)

Li, N., Kunitake, E., Aoyama, M., Ogawa, M., Kanamaru, K., Kimura, M., Koyama, Y., and Kobayashi, T., McmA-dependent and -independent regulatory systems governing expression of ClrB-regulated cellulase and hemicellulase genes in Aspergillus nidulans, Mol. Microbiol., 102(5) 810-826 (2016).

Kojima, T., Kunitake, E., Ihara, K., Kobayashi, T., and Nakano, H., A robust analytical pipeline for genome-wide identification of the genes regulated by a transcription factor: combinatorial analysis performed using gSELEX-Seq and RNA-Seq, PLoS ONE, 11 (7) e0159011 (2016).

Kunitake, E., Hagiwara, D., Miyamoto, K., Kanamaru, K., Kimura, M., and Kobayashi, T., Regulation of genes encoding cellulolytic enzymes by Pal-PacC signaling in Aspergillus nidulans, Appl. Microbiol. Biotechnol., 100 (8) 3621-3635 (2016).

Li, N., Kunitake, E., Endo, Y., Aoyama, M., Kanamaru, K., Kimura, M., Kato, M., and Kobayashi, T., Involvement of an SRF-MADS protein McmA in regulation of extracellular enzyme production and asexual/sexual development in Aspergillus nidulans, Biosci. Biotechnol. Biochem., 80 (9) 1820-1828 (2016).

Kunitake, E., Kawamura, A., Tani, S., Takenaka, S., Ogasawara, W., Sumitani, J., and Kawaguchi, T., Effects of clbR overexpression on enzyme production in Aspergillus aculeatus vary depending on the cellulosic biomass-degrading enzyme species, Biosci. Biotechnol. Biochem., 79 (3) 488-495 (2015).

Tani, S., Tsuji, A., Kunitake, E., Sumitani, J., and Kawaguchi, T., Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus, AMB Express, 3 (1) 4 (2013).

Kunitake, E., Tani, S., Sumitani, J., and Kawaguchi, T., A novel transcriptional regulator, ClbR, controls the cellobiose- and cellulose-responsive induction of cellulase and xylanase genes regulated by two distinct signaling pathways in Aspergillus aculeatus, Appl. Microbiol. Biotechnol., 97 (5) 2017-2028 (2013).

Kunitake, E., Tani, S., Sumitani, J., and Kawaguchi, T., Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis, AMB Express, 1 (1) 46 (2011).

総説等

國武絵美,小林哲夫,糸状菌における植物バイオマス分解酵素遺伝子の発現制御, JSM Mycotoxins, 66 (1) 85-96 (2016).

國武絵美,谷修治,炭谷順一,川口剛司,糸状菌セルラーゼ遺伝子群の転写活性化機構の解析, JSM Mycotoxins, 64 (1) 45-54 (2014).

Kunitake, E., Tani, S., Sumitani, J., and Kawaguchi, T., Development of Agrobacterium tumefaciens-meditated transformation system for random insertional mutagenesis in Aspergillus aculeatus, Biotechnology of Lignocellulose Degradation and Biomass Utilization (Proceedings of Mie Bioforum 2008, ISBN 978-4-9903-219-6-3 C-3845), 521-526 (2008).

Tsuji, A., Kunitake, E., Tani, S., Sumitani, J., and Kawaguchi, T., Development of highly efficient gene-targeting system by disrupting Aspergillus aculeatus ku80 gene, Biotechnology of Lignocellulose Degradation and Biomass Utilization (Proceedings of Mie Bioforum 2008, ISBN 978-4-9903-219-6-3 C-3845), 549-554 (2008).