In our laboratory, we have acquired the techniques of chemically analyze for soil and plants, and are conducting research on the adaptation of plants to the growing soil and the mechanism of excess and deficiency for each element. In recent years, we have been conducting research on nickel-iron nutrition in serpentinite soil, which is characterized by oligotrophic and high metal. Now we have started analysis of plant specimens owned by museums and the like. , We are proceeding the construction of an "element accumulation database" for wild plants in Japan, using XRF analyze method and plant specimens of museums.